Research papers

shaimaa zayed

Sana Maher Aghbari

2/9/2018

Evaluating the role of tissue microRNA‐27b as a diagnostic marker for oral lichen planus and possible correlation with CD8

CD8, cytotoxic T cells, oral lichen planus

Background: MicroRNA‐27b (miR27b) is a small, non‐coding RNA that is involved in physiological keratinocyte differentiation and regulating inflammatory processes. We performed this study to investigate the value of miR27b as a diagnostic marker for oral lichen planus (OLP) and the correlation between CD8 (cytotoxic T‐cell marker) and miR27b tissue expression in OLP patients. Methods: Forty participants (including 20 OLP patients and 20 controls) underwent oral biopsy. The obtained specimens were examined by immunostaining and quantitative RT‐PCR for CD8 and miR27b tissue expression, respectively. We used the Spearman rank correlation test to evaluate the correlation between both variables. Results: Our analysis showed that in comparison with healthy tissues, OLP tissue samples exhibited significantly higher CD8 levels (P < 0.01), as well as a significant downregulation of miR27b expression (P < 0.0001). Upon comparing different OLP subgroups, no significant difference was detected in terms of miR27b expression; however, the tissue levels of CD8 varied significantly (highest in the erosive subgroup and lowest in the papular/plaque/reticular subgroup). The Spearman rank analysis showed a negative correlation between tissue expression of miR27b and CD8; however, this was not statistically significant (P > 0.05). Further, the receiver operating characteristic curve of tissue miR27b as an OLP biomarker revealed 100% sensitivity and 65% specificity at cutoff value of 4.4. Conclusion: This study demonstrated increased CD8 levels and downregulation of miR27b in OLP tissues, compared to healthy tissues. Moreover, it revealed the potential of miR27b as an OLP disease biomarker. The possible negative correlation between CD8 and miR27b tissue expression requires further investigation in larger studies.

Sana Maher Aghbari

2/9/2018

Evaluating the role of tissue microRNA‐27b as a diagnostic marker for oral lichen planus and possible correlation with CD8

CD8, cytotoxic T cells, oral lichen planus

Background: MicroRNA‐27b (miR27b) is a small, non‐coding RNA that is involved in physiological keratinocyte differentiation and regulating inflammatory processes. We performed this study to investigate the value of miR27b as a diagnostic marker for oral lichen planus (OLP) and the correlation between CD8 (cytotoxic T‐cell marker) and miR27b tissue expression in OLP patients. Methods: Forty participants (including 20 OLP patients and 20 controls) underwent oral biopsy. The obtained specimens were examined by immunostaining and quantitative RT‐PCR for CD8 and miR27b tissue expression, respectively. We used the Spearman rank correlation test to evaluate the correlation between both variables. Results: Our analysis showed that in comparison with healthy tissues, OLP tissue samples exhibited significantly higher CD8 levels (P < 0.01), as well as a significant downregulation of miR27b expression (P < 0.0001). Upon comparing different OLP subgroups, no significant difference was detected in terms of miR27b expression; however, the tissue levels of CD8 varied significantly (highest in the erosive subgroup and lowest in the papular/plaque/reticular subgroup). The Spearman rank analysis showed a negative correlation between tissue expression of miR27b and CD8; however, this was not statistically significant (P > 0.05). Further, the receiver operating characteristic curve of tissue miR27b as an OLP biomarker revealed 100% sensitivity and 65% specificity at cutoff value of 4.4. Conclusion: This study demonstrated increased CD8 levels and downregulation of miR27b in OLP tissues, compared to healthy tissues. Moreover, it revealed the potential of miR27b as an OLP disease biomarker. The possible negative correlation between CD8 and miR27b tissue expression requires further investigation in larger studies.

shaimaa zayed

Reham Salah hamed

7/16/2021

COMPARING EFFECT OF ANNONACEAE AND EMETINE NANOPARTICLES ON NFĸB p65 PATHWAY IN TONGUE SQUAMOUS CELL CARCINOMA (scc-25)

Annonaceae, Emetine drug, chitosan nanoparticles, NFkB65.

Objective: The transformed cells in oral cancer secrete inflammatory mediators which generate an inflammatory microenvironment result in malignant transformation. Nuclear factor-κB (NF-κB) regulates a large number of genes which play a critical roles of the immune, inflammatory responses, cell proliferation, differentiation, and survival. Nanoparticles are 100 to 10,000 times smaller than human cells, could be designed to be highly selective for tumors and allow a slow release of active anticancer agents. We performed this study to investigate the effect of annonaceae nanoparticles and Emetine/Chitosan nanoparticles against scc-25 cell lines human tongue squamous cell carcinoma by studying their effect on NF-κB p65 expression. Methods: Cell Line cells were obtained from American Type Culture Collection, cells were cultured using DMEM (Invitrogen/Life Technologies). The effect of Annonaceae nanoparticles and EMETIN drug were investigated by Enzyme-Linked Immunosorbent Assay. The Signal is generated proportionally to the amount of bound NFĸB p65 and the intensity is measured by ROBONIK P2000 ELISA READER at 450 nm. Results: Treatment of tongue scc-25 cells with both annonacea NP and Emetine/Chitosan NP revealed a significant increase in expression of NFĸB p65 in cancer cells by (227 & 261 pg/ml) respectively at incubation period 48 hours . While the control untreated cancer cells scc-25 revealed lower expression of NFĸB p65 at (147 pg/ml at incubation period 48 hour. Conclusion: Our findings demonstrate that annonaceae and Emetine nanoparticle might have regulatory effect on canonical NF-kb pathway by upregulation of NFĸB p65 expression signals.

shaimaa zayed

7/15/2021

CORRELATION BETWEEN DEGREE OF PULP INFLAMMATION AND SUCCESS RATE OF MTA PULPOTOMY IN PRIMARY TEETH: PROSPECTIVE COHORT STUDY

Cytokines, vital pulp therapy, caries level, pulp inflammation. ELISA

Objective: Many studies reported that the subjective clinical and radiographic examination lack the accuracy about the biological status of the pulp tissue. cytokines produced by dental pulp cells may reflect the degree of pulpal inflammation. The purpose of this study is to Correlate the cytokine levels of (IL-1α, IL-6, and IL-8) present in coronal dental pulp of primary molars with the success rate of MTA based vital pulpotomy over 18 months follow up period. Methods: 40 primary molars were enrolled. The cases are divided into group A (caries level code 5), group B (caries level code 6). Blood and pulp tissue were collected from the pulp cavity by sterile cotton pellet. The pellet was placed in 0.5ml (500 µl) phosphate buffered saline solution (PBS) to 1/10 final volume in a heparin-coated tube. Levels of cytokines IL-1 α, IL-6 and IL-8 were measured by using ELISA. Results: We found that IL-1 α, IL-6 and IL-8 were significant higher in group (B) than in (A). IL-6 showed the most significant value of expression in comparison to IL-1 α and IL-8 (p-value: 0.011, 0.014 & 0.027 respectively) which denote the prominent role of IL-6 during the inflammatory process in the pulp tissue of primary molars. Conclusion: IL-1 α, IL-6 and IL-8 cytokine expression in primary pulp molars increase with caries progression. Although the significant difference in the cytokine level expression between group A and B, a high success rate of MTA vital pulpotomy was found during the follow up period in both groups.

Basant Hamdy

Omneya Wahba

Heba Youssef

12/1/2022

Mammaglobin immunoexpression in some malignant salivary gland neoplasms as a potential diagnostic tool for tumor ductal differentiation

ductal differentiation

immunohistochemical study

mammaglobin

salivary gland neoplasms

Introduction: Despite being a rare daily histopathologic diagnosis, malignant salivary gland (SG) tumors show significant morphologic diversity that could obscure their diagnosis. Recognizing specific microscopic patterns together with the aid of ancillary techniques, such as immunohistochemistry, represent the clues to precise diagnosis. Mammaglobin (MMg) is a member of secretoglobin protein family that is widely associated with breast cancer and strongly expressed by secretory carcinoma of SGs. Its expression in other malignant SG neoplasms should be investigated. Objectives Investigating the immunohistochemical expression of MMg in some malignant SG tumors to detect its potential diagnostic value. Material and Methods: Nineteen paraffin-embedded, archival specimens of malignant SG tumors were divided into two groups according to presence or absence of ductal differentiation. Specimens were immunohistochemically stained using anti-MMg protein. Results: MMg expression was more upregulated in the ductal differentiation group as compared to the other group of neoplasms. Conclusion: MMg would represent a promising diagnostic tool in discriminating SG tumors with ductal and/or secretory activity.

shaimaa zayed

shaimaa zayed

Marwaa refaat

12/3/2023

EXPRESSION AND CORRELATION OF MICRORNA-34C AND CD68 IN DIFFERENT GRADES OF ORAL SQUAMOUS CELL CARCINOMA VERSUS NORMAL ORAL MUCOSA

Keywords: OSCC;miRNA-34c; RT-Qpcr; Mir-34 family,TAMs,CD68

Background: Oral cancer is one of the most prevalent cancers worldwide, with squamous cell carcinomas being the most common type, accounting for 90% of all oral cancers with high recurrence in many cases. Thus, the development of new methods to predict the prognosis of OSCC is essential. Aim: To investigate and correlate the expression profile of miR-34c using RT-qPCR and quantify CD68 antibody usingimmunohistochemistry in normal oral mucosal tissues versus different grades of OSCC.Methodology: Cases of oral squamous cell carcinomas were retrieved from archival blocks between 2016 to 2021, from the Department of Oral and Maxillofacial Pathology, Faculty of dentistry.Results: All grades of OSCC revealed significant lower mean expression of miR-34c in comparison to normal mucosal tissue samples at p-value (0.000) with the lowest expression noticed in well differentiated OSCC at p-value (0.000).CD68+ Macrophages in each group of OSCC were found to be significantly higher compared to the normal oral mucosa with the highest expression seen at (WellModerate group) at p-value of (0.000).

Abdelrahman Sharaf eldeen

4/12/2023

Transduction of miRNA-155 and miRNA-34a in Oral Squamous Cell Carcinoma Cell Line

OSCC CELL LINE miRNA

Objective: to assess the impact of miRNA-155 and miRNA-34a suppression and replacement on the possibility of human Tongue squamous cell carcinoma (SCC) cell line (HNO97). Design: In vitro study. Setting: Global Research Labs, Medical Center2, Nasr City, Cairo, Egypt Interventions: The HNO97 cells were transfected with miR-155 and miR-34a imitates and inhibitors, then the cell capability was assessed using the MTT assay, and the c-Myc and CDK6 genes expression was calculated in treated and untreated cells using SYBER green based quantitative polymerase chain reaction. Main outcome measure: To detect how transduction of oral SCC (OSCC) cell line with miRNA-155 and miRNA-34a mimic and inhibitor affects the expression of c-Myc and CDK6 genes. Results: The cell viability was precisely as well as directly linked with the expression of miR-155 and the case was the opposite when miRNA-34a was used, in addition, higher expression level of c-Myc gene was combined with miR-155 mimic and miR-34a suppression and, the CDK6 gene expression showed an increase in cells treated with miR-155 mimic as well as miR-34a mimic and inhibitor. Conclusions: miR-155 has a significant oncogenic effect on OSCC cell lines by increasing the c-Myc and CDK6 gene expression, but miR-34a lacks that well defined effect

Abdelrahman Sharaf eldeen

5/9/2019

Liposome-coated nano doxorubicin induces apoptosis on oral squamous cell carcinoma CAL-27 cells

Oral squamous cell carcinoma Doxorubicin Doxil Polyethylene glycol nano-carriers

Objective: This study aims to investigate the apoptotic effect of Doxorubicin and its nano-formulated form (Doxil) on oral squamous cell carcinoma CAL-27 cells. Design: Cell viability using MTT assay, mode of cell death using fluorescence analysis, expression of the apoptotic marker caspase-3 using indirect ELISA technique and expression of C-Myc gene using reverse transcriptase and real time PCR. Results: Doxil treatment resulted in a higher percentage of apoptotic cells than doxorubicin treatment, while doxorubicin treatment resulted in a higher percentage of necrotic cells than Doxil treatment. Doxil-treated cells exhibited 3.38-fold higher caspase-3 levels than control cells, while doxorubicin significantly increased caspase3 levels by 2.72-fold. The percentage of C-Myc mRNA inhibition was 27% in doxorubicin-treated cells and 41% in Doxil-treated cells. Conclusions: Doxil exerted a higher apoptotic effect on CAL-27 cells compared to doxorubicin. It showed a higher increase in capase-3 level than doxorubicin and also exerted a more percentage of C-Myc mRNA inhibition.

Abdelrahman Sharaf eldeen

5/6/2022

Detection and Suppression of Survivin (BIRC5) in Oral Squamous Cell Carcinoma Cell Line: An Invitro Study

OSCC SURVIVIN

(1) Background: In the current study, we aimed to assess the effect of suppression of survivin (BIRC5) on the viability of oral squamous cell carcinoma cell line and to explore the changes in the expression of both p53 and bcl2 genes when surviving was blocked; (2) Methods: The oral squamous cell carcinoma cell line (HNO97) cells were transfected with si- BIRC5 then the cell viability was evaluated using the MTT assay. In addition, we investigated the effect of silencing BIRC5 as an apoptotic inhibitor protein on two apoptosis-regulating genes TP53 and BCL2. (3) Results: There was a marked decrease in cell viability when cell line was transfected with si- BIRC5, in addition to that, unlike Bcl2 which showed a marked decrease, the expression of p53 was increased

Basant Hamdy

Shaden A.Hammad

Najla Dar-Odeh

+4

3/31/2020

Influence of gender on career expectations of oral and maxillofacial surgeons

Satisfaction

Mentorship

career expectations

Oral surgeon

Objectives This study aimed to explore gender differences on career satisfaction among oral and maxillofacial surgeons (OMFS) and to compare their views on female gender representation in leadership and mentorship positions. Methods A questionnaire was distributed among a sample of female and male OMFS surgeons in Egypt, Jordan and Saudi Arabia. Data were statistically analyzed to calculate significant differences between gender groups. Results A total of 110 (70 males and 40 females) or (18.7%) of a total of 587 (OMFS) surgeons in the three countries participated. Respondents had a mean age of: 39.2 ± 9.0 years (range = 25–60 years). Significantly more males (60) than females (24) indicated satisfaction with their careers (P = 0.008), and significantly more males (66) than females (23) thought that OMFS is the right specialty for them (P < 0.001). Also significantly more males (31) than females (9) indicated OMFS should be solely practiced by males (P = 0.025). On the other hand, significantly more females (20) than males (7) thought that females possess certain qualities that make them more suitable for OMFS (P < 0.001); and more females (27) than males (32) believed that females are under-represented in OMFS (P = 0.031). Significantly more females (25) than males (27) believed that female OMFS surgeons tend to be single or to get divorced (P = 0.018). Males and females were in agreement that females are under-represented in leadership and mentorship positions (P = 0.561). Conclusions Females OMFS surgeons are less satisfied with their career than their male peers. It seems that female surgeons are under-represented in the profession especially as leaders and mentors. Socially, more female OMFS surgeons than males tend to be single and this may be correlated with the surgical career.